Effect of L-Arginine on sperm Quality of Rainbow Trout (Oncorhynchus mykiss)”,

L-arginine is a versatile amino acid and precursor of nitric oxide (NO). NO provides protection for preventing free radical damage. Herein, experiments were designed to clarify the effect of L-arginine supplementation on sperm motility of rainbow trout, Oncorhynchus mykiss. We used to levels of 0 mM (Control), 1 mM, 2 mM, 4 mM and 8 mM L-arginine. To assess the effects on motility and survival of sperm cells, L-arginine was added to activation medium containing NaCl (52 mM). The higher L-arginine concentration (4 mM) has promoting-effect on sperm motility. Significant effect of L-arginine supplementation was determined on the percentage and duration of motile spermatozoa (p<0.05). Here, we showed that addition of L-arginine can improve sperm motility of O. mykiss

The Reproduction Traits of Native Brown Trout Salmo trutta macrostigma T 1954 Turkey

Some key aspects of the reproductive strategy of the brown trout Salmo trutta macrostigma (T., 1954) inhabiting Uzungol stream, Trabzon including spawning season, age at sexual maturity, fecundity and egg size have been studied between January 2009 to May 2010. The ages of the captured fish ranged from 2-8 years. Individuals were composed of 40.85% males and 37.80% females. The present investigation on the fecundity of S.t. macrostigma was carried out to estimate the average and range in the number of ova laid by individual female as well as to study the relationship of the fecundity with total length, total body weight. The mean value of absolute fecundity was 312.23±254.71 (SD) eggs/female. The regression between egg number and fish size (total length and weight) was statistically significant (p<0.05)

Effect of dietary kefir on the digestive and liver enzymes activities and glucose level of Coruh trout Salmo coruhensis Actinopterygii Salmoniformes Salmonidae.

The probiotics are very important for aquaculture because of their role in enhancing the fish health status, improving disease resistance, growth performance, and body composition, reducing malformations, improving gut morphology and the overall microbial balance. The objective of the presently reported study was to investigate effects of kefir as a probiotic product on the digestive and hepatic enzyme activities, as well as glucose level of Coruh trout, Salmo coruhensis Turan, Kottelat et Engin, 2010. The experiment was performed with four treatments: control group (no kefir), D1, D2, and D3 (kefir-supplemented diet: 10, 20, and 40 g . kg(-1) fish body mass, respectively). The activity of: alanine aminotransferase (ALT), aspartate aminotranferase (AST), lactate dehydrogenase (LDH), lipase, amylase-as well as the glucose levels were determined after 4 months. The results indicated that glucose levels (P 0.05). On the contrary, the activity of liver enzymes (ALT, AST, and LDH) increased insignificantly (P > 0.05). In conclusion, digestive and liver enzyme activities were affected by quantitative changes in dietary kefir insignificantly but further research is needed to establish whether the liver and digestive enzyme physiology of Coruh trout can be affected by kefir-supplemented diets in a long-term trial

Determination of Bioaccumulation of Heavy Metals and Selenium in Tissues of Brown Trout Salmo trutta macrostigma Duméril 1858 from Munzur Stream Tunceli Turkey

The objective of the present work was to determine the bioaccumulation of arsenic (As), cadmium (Cd), copper (Cu), lead (Pb), mercury (Hg), uranium (U) and selenium (Se) in gill, liver, and muscle tissues of the fresh water fish Salmo trutta macrostigma (Duméril, 1858) in Munzur Stream, Tunceli, Turkey. The highest concentrations of U (1.83 μg kg−1), Pb (119.84 μg kg−1) and Se (1.31 μg kg−1) were recorded in the gills of S. t. macrostigma. Concentrations of As (46.27 μg kg−1), Cd (109.19 μg kg−1), Hg (16.40 μg kg−1), Cu (18.19 μg kg−1) were recorded at highest levels in the liver. The results showed that there were significant differences in concentrations of As, Cd, Cu, Pb, Se, U and Hg in gill, liver and muscle tissue (p < 0.05). Heavy metals were within the edible parts of the investigated fish were in the permissible safety levels for human uses

Effect of dietary n 3 series fatty acids on sperm production in the freshwater crayfish Astacus leptodactylus Eschscholtz Astacidae

The objective of the present work was to evaluate the effects of three different rates of long chain n-3 polyunsaturated fatty acid (LC n-3 PUFA) on the sperm number of Astacus leptodactylus. The experiment was carried out with the four following treatments: control group (not supplemented LC n-3 PUFA), D1 (1 %), D2 (2 %) and D3 (3 %). The sperm number of A. leptodactylus was counted; the reproductive system was weighted; and the macroscopical analysis of the testes and vasa deferentia (DVD) were conducted. In addition, gonado-somatic index (GI) and testes index (TI) were also calculated. The results showed that there was a significant difference between the crayfish fed LC n-3 PUFA supplemented diets (D1, D2, D3) and control in mean sperm number (P 0.05), but there was not a significant difference in the TI of the crayfish fed control, D1, D2 and D3 (P > 0.05). In conclusion, diets containing different levels of LC n-3 PUFA significantly increase the sperm number of A. leptodactylus, and they are crucial for the production of higher number of sperm in this species